Determination of Anticyclic Citrullinated Peptide Based on Biotin-Streptavidin-Amplified Time-Resolved Fluoroimmunoassay
نویسندگان
چکیده
منابع مشابه
Determination of thyrotropin in serum by time-resolved fluoroimmunoassay evaluated.
We evaluated a new, highly sensitive time-resolved fluoroimmunoassay for thyrotropin (TSH) in serum. This direct immunometric "sandwich"-type assay involves two monoclonal antibodies against TSH, one immobilized, the other labeled with europium. Extremely high specific activity of the label and the use of labeled antibody in large excess make the method sensitive enough to measure TSH values fa...
متن کاملSensitive time-resolved fluoroimmunoassay of salmon calcitonin.
A two-site assay was developed by use of the "dissociation and enhancement lanthanide fluoroimmunoassay" (DELFIA) technique for determination of salmon calcitonin (SCT) in serum after administration to osteoporotic patients. Polyclonal antibodies were produced in rabbits immunized with SCT coupled to ovalbumin. After affinity purification, the antibodies were used both as immobilized capture an...
متن کاملAn enantioselective artificial Suzukiase based on the biotin–streptavidin technology
Introduction of a biotinylated monophosphine palladium complex within streptavidin affords an enantioselective artificial Suzukiase. Site-directed mutagenesis allowed the optimization of the activity and the enantioselectivity of this artificial metalloenzyme. A variety of atropisomeric biaryls were produced in good yields and up to 90% ee. The hybrid catalyst described herein shows comparable ...
متن کاملAnticyclic Citrullinated Peptide Antibody: A Predictor of Juvenile Idiopathic Arthritis Progression Toward Rheumatoid Arthritis
Rheumatoid Arthritis (RA) has been associated with several autoantibodies, including antiperinuclear factor, anti-keratin antibodies, anti-filaggrin antibodies, and anti-Cyclic Citrullinated Peptide (anti-CCP) antibodies. Most studies found that anti-CCP antibodies are only detected in Juvenile Rheumatoid Arthritis (JRA) patients with positive Rheumatoid Factor (RF). In our center we experiment...
متن کاملBiotin-4-Fluorescein Based Fluorescence Quenching Assay for Determination of Biotin Binding Capacity of Streptavidin Conjugated Quantum Dots
The valency of quantum dot nanoparticles conjugated with biomolecules is closely related to their performance in cell tagging, tracking, and imaging experiments. Commercially available streptavidin conjugates (SAv QDs) are the most commonly used tool for preparing QD-biomolecule conjugates. The fluorescence quenching of biotin-4-fluorscein (B4F) provides a straightforward assay to quantify the ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Clinical Laboratory Analysis
سال: 2014
ISSN: 0887-8013
DOI: 10.1002/jcla.21796